Low-Shift Raman Microscope Attachment
Low-Shift Raman Microscope Attachment

Low-Shift Raman Microscope Attachment

This project involves designing a microscope attachment that can create and detect a Raman signal for samples exhibiting characteristics of producing strong Raman scattering. Raman microscopy allow for chemists, forensic scientist, and engineers to characterize materials given a spectrum. The sections of this project includes optics to produce Raman excitation and a spectrometer to detect the Raman signal. Electronics will be integrated into the system to produce safety measures, temperature control, lighting control, and light signal detection. Along with electronics and optics, software is included to control the system and display system characteristics through a Graphical User Interface (GUI).

Summary

What does the project do?

This device integrates a microscope with a Raman spectroscopy system that can easily provide a Raman spectrum for a given material.

Who needs it/who would use it?

Our sponsor, Dr. Matthieu Baudelet, is a professor of Chemistry at the National Center of Forensic Science (NCFS) that needs a Raman spectroscopy system in his lab to analyze materials such as pollen, expolsive powders, and drugs. The Raman system has to be easy to use and safe for chemistry students as well.

How does it work?

The Raman spectroscopy system is used by first placing a sample of interest on the microscope stage. Then the z-position of the sample stage is moved closer to the microscope objective to a positon where the sample is focused on the camera image software. When the camera is focused on the sample, the laser is focused on the sample to induce Raman scattering. By hitting the "create Raman spectrum" button on the GUI will allow the CCD to capture and display a Raman spectrum via software.

Why is it different/ better than what's available or has been done before?

This Raman spectroscopy system is built with a microscope to allow for easy sampling, sample imaging, and strong laser focusing to generate a Raman signal at a detectable intensity. Not a lot of companies sell Raman Spectroscopy systems that is integrated with a microscope and if there is, it is over $70,000.

Our Sponsor:

Our sponsor is Dr. Matthieu Baudelet, who is a professor of Chemistry at the National Center of Forensic Science (NCFS). He is passionate about laser spectroscopy and applying the technique to benefit the scientific comunnity and student education. He has not only provided financial support, but also a quiet and spacious lab to work in with enough supplies and equipment to get the project going. Above all, he provided encourgement, endless advice, support, and was there for this group throughout the entire senior design semester. Thank you Dr. Matthieu for placing your faith in this senior design group even when we didn't believe in ourselves and for helping us graduate.